Lung cancer has posed a particular challenge to medical researchers; though there are patients that can serve as test subjects during clinical trials, such trials take months, if not years to complete. Such issues have been resolved through the advent of cell lines that accurately replicate those that would be found in tumors, and can react appropriately to introduced drugs and nucleic acid sequences. With the right transfection reagents, research investigating the role that genetic mechanisms play in cell proliferation can give accurate data that translates into valuable preclinical information. Calu-6 cells, which are epithelial lung cancer cells, were derived from human tissue, and are known for having been used to gauge the interaction between antimycin A and lung cancer, giving the medical field information regarding the effects of reactive oxygen species on cell apoptosis.
The Calu-6 cell line was established from the lung tissue of a 61-year-old Caucasian female who had been suffering from anaplastic carcinoma. Calu-6 cells are tumorigenic and have a modal chromosome number of 59. They also exhibit adherent cultural properties and display an epithelial morphology. The Calu-6 cell line is a suitable transfection host for in vivo and in vitro methods to study lung cancer, and a Calu-6 Transfection Reagent can be purchased through Altogen Biosystems to transfect Calu-6 cells. A Calu-6 xenograft model can be found here .
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