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Lung Cancer Research With Calu-6 Cells and Transfection

Lung cancer has posed a particular challenge to medical researchers; though there are patients that can serve as test subjects during clinical trials, such trials take months, if not years to complete. Such issues have been resolved through the advent of cell lines that accurately replicate those that would be found in tumors, and can react appropriately to introduced drugs and nucleic acid sequences. With the right transfection reagents, research investigating the role that genetic mechanisms play in cell proliferation can give accurate data that translates into valuable preclinical information. Calu-6 cells, which are epithelial lung cancer cells, were derived from human tissue, and are known for having been used to gauge the interaction between antimycin A and lung cancer, giving the medical field information regarding the effects of reactive oxygen species on cell apoptosis.

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Transfection and Xenograft Tools for Calu-6 From Altogen Biosystems

Altogen Biosystems is an international developer and laboratory for genetic research. Laboratory offers and additional experiments include IC-50 assays, toxicology assays, 28-day generation of stably-transfected cells, RNAi testing and more. Dedicated to aiding pharmaceutical companies with cutting-edge and specialized services, Altogen Biosystems ventures to optimize modern laboratory discoveries. Visit the catalog of xenograft models for a Calu-6 xenograft model .

Calu-6 Cell Line

The Calu-6 cell line was established from the lung tissue of a 61-year-old Caucasian female who had been suffering from anaplastic carcinoma. Calu-6 cells are tumorigenic and have a modal chromosome number of 59. They also exhibit adherent cultural properties and display an epithelial morphology. The Calu-6 cell line is a suitable transfection host for in vivo and in vitro  methods to study lung cancer, and a Calu-6 Transfection Reagent  can be purchased through Altogen Biosystems  to transfect Calu-6 cells. A Calu-6 xenograft model can be found here . 

The Role of Vandetanib in Calu-6 Cells

Vandetanib is a multikinase inhibitor that is a target of drug treatments for non-small cell lung cancer. In this study, researchers determine the cellular and biological effects of vandetanib on Calu-6 cells. Results showed that vandetanib inhibits Calu-6 cell migration and invasiveness. Vandetanib also induces autophagy in Calu-6 cells by increasing the level of reactive oxygen species (ROS). These findings show that vandetanib produces a double effect in some NSCLC cells, which presents vandetanib as a potential agent for treatment of Calu-6 cells. [ LINK ]