Transfection Reagent for Calu-6 Cells (Lung Carcinoma Cell, HTB-56)

Vandetanib is a multikinase inhibitor that is a target of drug treatments for non-small cell lung cancer. In this study, researchers determine the cellular and biological effects of vandetanib on Calu-6 cells. Results showed that vandetanib inhibits Calu-6 cell migration and invasiveness. Vandetanib also induces autophagy in Calu-6 cells by increasing the level of reactive oxygen species (ROS). These findings show that vandetanib produces a double effect in some NSCLC cells, which presents vandetanib as a potential agent for treatment of Calu-6 cells. [ LINK ]

Tumors are characterized by either tumor vessel or stromal vessel phenotypes, which reportedly define how tumors responds to VEGFR2 antibody treatment. In this study, researchers seek to understand whether the tumor phenotypes associate with vascular response to VEGFR tyrosine kinase inhibitors (TKI) or if changes in vascular function are associated with changes in tumor size. Stromal vessel phenotype Calu-3 and tumor vessel phenotype Calu-6 cells were treated with cediranib for 5 days. Following treatment, cediranib decreased Calu-3 tumor perfusion and hypoxia significantly increased; however, neither of these factors were affected in Calu-6 tumors. These findings suggest that tumor stromal phenotypes associate more with acute tumor vascular response to VEGFR TKI. [ LINK ]

Curcumin is an herbal compound that has been reported to decrease telomerase and gene expression and could be used to treat lung cancer. In this study, researchers analyze the effects of pure curcumin and curcumin-loaded NIPAAm-MAA nanoparticles on Calu-6 cells. Real-time PCR was used to measure telomerase and pinX1 gene expression. The PCR results revealed that curcumin decreased the expression of telomerase gene and the expression of pinX1 increased. An MTT assay revealed that curcumin-loaded NIPAAm-MAA inhibited the growth of Calu-6 cells in a dose-dependent manner. These findings revealed that both types of curcumin produced cytotoxic effects on Calu-6 cells through the down-regulation of telomerase and stimulation of pinX1 gene expression. [ LINK ]

Altogen Biosystems is an international developer and laboratory for genetic research. Laboratory offers and additional experiments include IC-50 assays, toxicology assays, 28-day generation of stably-transfected cells, RNAi testing and more. Dedicated to aiding pharmaceutical companies with cutting-edge and specialized services, Altogen Biosystems ventures to optimize modern laboratory discoveries. Visit the catalog of xenograft models for a Calu-6 xenograft model .

Lung cancer has posed a particular challenge to medical researchers; though there are patients that can serve as test subjects during clinical trials, such trials take months, if not years to complete. Such issues have been resolved through the advent of cell lines that accurately replicate those that would be found in tumors, and can react appropriately to introduced drugs and nucleic acid sequences. With the right transfection reagents , research investigating the role that genetic mechanisms play in cell proliferation can give accurate data that translates into valuable preclinical information. Calu-6 cells, which are epithelial lung cancer cells, were derived from human tissue, and are known for having been used to gauge the interaction between antimycin A and lung cancer, giving the medical field information regarding the effects of reactive oxygen species on cell apoptosis.

The Calu-6 (ATCC number HTB-56) epithelial cell line was derived from the tissue of a 61-year-old Caucasian female suffering from anaplastic carcinoma (lung cancer). The Calu-6 transfection cells are used for research models to further study human lung cancer and its leading pathways. Additionally, research on the relationship between  animycin A (AMA) and  lung cancer cells have also been conducted.  Calu-6 transfection reagents  enhance the intracellular delivery of oligonucleotides when the Calu-6 cell line is used as a transfection host.  Transfection Reagent for Calu-6 Cells (Lung Carcinoma Cell, HTB-56)