The Calu-6 cell line was established from the lung tissue of a 61-year-old Caucasian female who had been suffering from anaplastic carcinoma. Calu-6 cells are tumorigenic and have a modal chromosome number of 59. They also exhibit adherent cultural properties and display an epithelial morphology. The Calu-6 cell line is a suitable transfection host for in vivo and in vitro methods to study lung cancer, and a Calu-6 Transfection Reagent can be purchased through Altogen Biosystems to transfect Calu-6 cells. A Calu-6 xenograft model can be found here .